Flow Cytometry

The LSR II analyser

The AMREP Flow Cytometry Core Facility is a state of the art, world class cell sorting and cell analysis laboratory, catering for the scientific research community based at AMREP and broader Melbourne.

The facility is located at AMREP on the Alfred Hospital site in Commercial Rd, Melbourne, specifically in the Central Clinical School (CCS) Monash ACBD (1st and 2nd floors), Monash Immunology 6th floor and the Burnet Institute (Alfred Centre 7th floor).

The facility is managed by Mr. Geza Paukovics, B.Med. Lab. Sci., with nearly 25 years of extensive experience in the field of flow cytometry and cell sorting. The facility offers comprehensive training and education, experimental design and protocol guidance specifically targeting effective data generation and interpretation in the field of flow cytometry. The facility offers services catering for both animal and human cell sorting in a PC2 environment. Uniquely, the facility also caters for infectious sample sorting (eg. HIV, HepC) in a dedicated PC3 environment. The facility is equipped with three cell sorting platforms (2x BD FACS Aria, 1x BD InFlux) and nine cell analysis platforms. (4x BD FACSCalibur, 2x BD FACS CantoII, 1X BD LSRII, 1x BD Fortessa, 1x BD Fortessa X-20) AMREPFlow also has acquired the AMNIS Imagestream MkII – an imaging flow cytometer, currently unique to AMREP and broader Melbourne. These instruments may only be booked by qualified and licensed users. All users must be familiar with the booking rules, associated usage costs, usage guidelines and standard operating procedures (SOPs). For more information please do not hesitate to contact us

AMREPFlow facility will be acquiring a 4th Cell Sorting Platform, the BD Fusion, with 6 excitation LASERs, inluding UV and Infra Red (IR). The estimated time of arrival for the BD Fusion SORP sorting platform will be around October 2017 onwards. The is a special order custom made instrument.

Staff members: AMREP Flow Manager: Mr. Geza Paukovics, Co-Manager: Eva Orlowski-Oliver C.Cy, Senior Flow Cytometrist: Magdaline Costa PhD